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Isolering av vilande stamcellspopulationer från enskilda skelettmuskler
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Isolering av vilande stamcellspopulationer från enskilda skelettmuskler

Article DOI: 10.3791/64557-v • 11:35 min • December 9th, 2022
December 9th, 2022 •
*1, *1, 1, 1, 1
1Department of Biomedicine, Aarhus University
* These authors contributed equally

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Detta protokoll beskriver isoleringen av muskelstamceller och fibro-adipogenic progenitorer från enskilda skelettmuskler hos möss. Protokollet innefattar dissektion av enstaka muskler, stamcellsisolering genom fluorescensaktiverad cellsortering, renhetsbedömning genom immunofluorescensfärgning och kvantitativ mätning av S-fasinträde med 5-etynyl-2'-deoxiuridininkorporeringsanalys.

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