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Cancer Research

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JoVE Journal Cancer Research
Engineering oncogene heterozygote gain-of-function mutaties in menselijke hematopoietische stam- en voorlopercellen
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Engineering oncogene heterozygote gain-of-function mutaties in menselijke hematopoietische stam- en voorlopercellen

Article DOI: 10.3791/64558-v • 12:04 min • March 10th, 2023
March 10th, 2023 •
1, 1, 2, 2, 1,3
1Division of Hematology, Department of Internal Medicine, Medical University of Graz, 2Division of Hematology, Stanford Cancer Institute, Stanford Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, 3Department of Blood Group Serology and Transfusion Medicine, Medical University of Graz

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Nieuwe strategieën om somatische mutaties in hematopoietische stam- en voorlopercellen (HSPC's) getrouw te modelleren, zijn nodig om de hematopoëtische stamcelbiologie en hematologische maligniteiten beter te bestuderen. Hier wordt een protocol beschreven om heterozygote gain-of-function mutaties in HSPC's te modelleren door het gebruik van CRISPR/Cas9 en dual rAAV donortransductie te combineren.

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Cancer Research CRISPR/Cas9 genome engineering leukemogenesis hematopoëtische stamcellen AAV mutatie homologe recombinatie gain-of-function mutaties
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