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Journal / Cancer Research / Entwicklung onkogener heterozygoter…
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Cancer Research

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JoVE Journal Cancer Research
Entwicklung onkogener heterozygoter Gain-of-Function-Mutationen in humanen hämatopoetischen Stamm- und Vorläuferzellen
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Entwicklung onkogener heterozygoter Gain-of-Function-Mutationen in humanen hämatopoetischen Stamm- und Vorläuferzellen

Article DOI: 10.3791/64558-v • 12:04 min • March 10th, 2023
March 10th, 2023 •
1, 1, 2, 2, 1,3
1Division of Hematology, Department of Internal Medicine, Medical University of Graz, 2Division of Hematology, Stanford Cancer Institute, Stanford Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, 3Department of Blood Group Serology and Transfusion Medicine, Medical University of Graz

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Neue Strategien zur getreuen Modellierung somatischer Mutationen in hämatopoetischen Stamm- und Vorläuferzellen (HSPCs) sind notwendig, um die hämatopoetische Stammzellbiologie und hämatologische Malignome besser zu untersuchen. Hier wird ein Protokoll zur Modellierung heterozygoter Gain-of-Function-Mutationen in HSPCs durch Kombination der Verwendung von CRISPR/Cas9 und dualer rAAV-Donortransduktion beschrieben.

Tags

Cancer Research CRISPR/Cas9 Genome Engineering Leukämie hämatopoetische Stammzellen AAV Mutation homologe Rekombination Gain-of-Function-Mutationen
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