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An Electroporation Method to Transform Rickettsia spp. with a Fluorescent Protein-Expressing Shuttle Vector in Tick Cell Lines
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An Electroporation Method to Transform Rickettsia spp. with a Fluorescent Protein-Expressing Shuttle Vector in Tick Cell Lines

Article DOI: 10.3791/64562-v • 07:18 min • October 11th, 2022
October 11th, 2022 •
1, 1, 1, 1
1Department of Entomology, University of Minnesota

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Electroporation is a rapid, broadly adopted method for introducing exogenous DNA into the genus Rickettsia. This protocol provides a useful electroporation method for the transformation of obligate intracellular bacteria in the genus Rickettsia.

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Electroporation Method Transform Rickettsia Spp. Fluorescent Protein-expressing Shuttle Vector Tick Cell Lines Functional Studies Pathogenesis Altering Genes Controlled Fashion Exogenous DNA Obligate Intracellular Bacteria Genus Rickettsia Effects Of Introduced DNA Giemsa Staining Infection Rate Cell Culture Preparation Microscope Slide Methanol Fixation Giemsa Stain Incubation Light Microscope Observation Infected Cells Percentage Determination Cell-free Rickettsia Parkeri
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