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JoVE Journal Neuroscience
Frazionamento subcellulare per l'isolamento di componenti sinaptici dal cervello murino
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Frazionamento subcellulare per l'isolamento di componenti sinaptici dal cervello murino

Article DOI: 10.3791/64574-v • 12:14 min • September 14th, 2022
September 14th, 2022 •
1,2,3, 1,2, 1,2
1Department of Neurology, Yale University, 2Department of Neuroscience, Yale University, 3Interdepartmental Neuroscience Program, Yale University

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Questo protocollo presenta un metodo robusto e dettagliato per ottenere sinaptosomi altamente puri, vescicole sinaptiche e altre frazioni sinaptiche dal cervello del topo. Questo metodo consente la valutazione dei processi sinaptici, compresa l'analisi biochimica della localizzazione e della funzione delle proteine con risoluzione compartimentale.

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Neuroscienze Numero 187
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