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Developmental Biology

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JoVE Journal Developmental Biology
Generering af naturlige dræberceller fra menneskelige stamceller med udvidet potentiale
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Generering af naturlige dræberceller fra menneskelige stamceller med udvidet potentiale

Article DOI: 10.3791/64608-v • 06:53 min • January 13th, 2023
January 13th, 2023 •
1, 2, 2, 2,3, 2,3
1Major in Molecular Biology and Biotechnology, School of Biological Sciences, The University of Hong Kong, 2School of Biomedical Sciences, The University of Hong Kong, 3Centre for Translational Stem Cell Biology

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Denne protokol viser, hvordan man kan differentiere CD3−/CD45+CD56+ celler med mild cytotoksicitet fra humane ekspanderede potentielle stamceller (hEPSC'er) under både 3D- og 2D-dyrkningsbetingelser. Dette giver mulighed for rutinemæssig fænotypisk validering uden ødelæggelse af det komplekse mikromiljø.

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Udviklingsbiologi udgave 191
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