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Developmental Biology

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JoVE Journal Developmental Biology
Geração de células assassinas naturais a partir de células-tronco de potencial expandido humano
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Geração de células assassinas naturais a partir de células-tronco de potencial expandido humano

Article DOI: 10.3791/64608-v • 06:53 min • January 13th, 2023
January 13th, 2023 •
1, 2, 2, 2,3, 2,3
1Major in Molecular Biology and Biotechnology, School of Biological Sciences, The University of Hong Kong, 2School of Biomedical Sciences, The University of Hong Kong, 3Centre for Translational Stem Cell Biology

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O presente protocolo mostra como diferenciar células CD3−/CD45+CD56+ com citotoxicidade leve de células-tronco potenciais expandidas humanas (hEPSCs) em condições de cultura 3D e 2D. Isso permite a validação fenotípica de rotina sem a destruição do microambiente complexo.

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Biologia do Desenvolvimento Edição 191
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