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JoVE Journal Developmental Biology
Generering av naturliga mördarceller från mänskliga expanderade potentiella stamceller
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Deriving Retinal Pigment Epithelium (RPE) from Induced Pluripotent Stem (iPS) Cells by Different Sizes of Embryoid Bodies
Journal (Developmental Biology)
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Generering av naturliga mördarceller från mänskliga expanderade potentiella stamceller

Article DOI: 10.3791/64608-v • 06:53 min • January 13th, 2023
January 13th, 2023 •
1, 2, 2, 2,3, 2,3
1Major in Molecular Biology and Biotechnology, School of Biological Sciences, The University of Hong Kong, 2School of Biomedical Sciences, The University of Hong Kong, 3Centre for Translational Stem Cell Biology

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Detta protokoll visar hur man differentierar CD3−/CD45+CD56+-celler med mild cytotoxicitet från humana expanderade potentiella stamceller (hEPSC) under både 3D- och 2D-odlingsförhållanden. Detta möjliggör rutinmässig fenotypisk validering utan förstörelse av den komplexa mikromiljön.

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Utvecklingsbiologi nummer 191
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