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JoVE Journal
Immunology and Infection

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A Rapid, Simple, and Standardized Homogenization Method to Prepare Antigen/Adjuvant Emulsions for Inducing Experimental Autoimmune Encephalomyelitis
 

A Rapid, Simple, and Standardized Homogenization Method to Prepare Antigen/Adjuvant Emulsions for Inducing Experimental Autoimmune Encephalomyelitis

Article DOI: 10.3791/64634-v 05:44 min December 9th, 2022
December 9th, 2022

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Summary

To induce experimental autoimmune encephalomyelitis, an animal model of multiple sclerosis, mice are immunized with a water-in-oil emulsion containing an autoantigen and complete Freund's adjuvant. While several protocols exist for the preparation of these emulsions, a rapid, simple, and standardized homogenization protocol for emulsion preparation is presented here.

Tags

Homogenization Method Antigen/adjuvant Emulsions Experimental Autoimmune Encephalomyelitis EAE Multiple Sclerosis Oil And Water Emulsion Consistent Results CFA Slurry M Tuberculosis H37 RA IFA Adjuvant CFA/Peptide Emulsion Screw Capped Tube Reagents PBS Peptide
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