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JoVE Journal Bioengineering
Three-Dimensional Culture of Vascularized Thermogenic Adipose Tissue from Microvascular Fragments
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Three-Dimensional Culture of Vascularized Thermogenic Adipose Tissue from Microvascular Fragments

Article DOI: 10.3791/64650-v • 11:24 min • February 3rd, 2023
February 3rd, 2023 •
1, 2,3, 2, 2, 2,3, 2,3
1Department of Biochemistry and Structural Biology, University of Texas Health Science Center, 2Department of Biomedical Engineering and Chemical Engineering, University of Texas at San Antonio, 3Institute of Regenerative Medicine, University of Texas at San Antonio

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Summary
Automatic Translation

Here, we present a detailed protocol outlining the use of microvascular fragments isolated from rodent or human fat tissue as a straightforward approach to engineer functional, vascularized beige adipose tissue.

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Three-dimensional Culture Vascularized Thermogenic Adipose Tissue Microvascular Fragments Engineering Multicellular Properties Functional Properties Beige Fat Obesity Metabolic Disease Animal Isolation Inguinal Fat Fascia Tissue Conical Tubes BSA In PBS Epididymal Fat
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