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Journal / Immunology and Infection / 유세포 분석 기술을 통한 일본 뇌염 백신 접종을받은 어린이의 다기능 T 세포…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
유세포 분석 기술을 통한 일본 뇌염 백신 접종을받은 어린이의 다기능 T 세포 검출
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유세포 분석 기술을 통한 일본 뇌염 백신 접종을받은 어린이의 다기능 T 세포 검출

Article DOI: 10.3791/64671-v • 09:37 min • September 23rd, 2022
September 23rd, 2022 •
*1,2, *1,2,3, 1,2, 1,2, 1,2, 4, 1,2, 1,2
1Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, 2Research Unit of Critical Infection in Children, 2019RU016, Chinese Academy of Medical Sciences, 3Department of Pediatric Rehabilitation, Beijing Boai Hospital, School of Rehabilitation Medicine, Capital Medical University, China Rehabilitation Research Center, 4Center of Excellence (Beijing), Becton Dickinson Medical Devices (Shanghai) Co Ltd
* These authors contributed equally

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본 프로토콜은 생체 외 자극과 유세포 분석을 결합하여 일본 뇌염 바이러스 (JEV) 예방 접종을받은 어린이 내의 말초 혈액 단핵 세포 (PBMC)에서 다기능성 T 세포 (TPF) 프로파일을 분석합니다. JEV 특이적 TPF의 검출 방법 및 유세포분석 색 구성표를 테스트하여 유사한 연구에 대한 참조를 제공했습니다.

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면역학 및 감염 187호 다기능성 T세포 일본뇌염 예방접종 유세포분석
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