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Journal / Genetics / Screening sperm for rask isolering av germline…
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Screening sperm for rask isolering av germline redigeringer i sebrafisk
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Screening sperm for rask isolering av germline redigeringer i sebrafisk

Article DOI: 10.3791/64686-v • 05:55 min • February 10th, 2023
February 10th, 2023 •
1, 2, 1,2
1Department of Molecular Biosciences, The University of Texas at Austin, 2Department of Nutritional Sciences, The University of Texas at Austin

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CRISPR-Cas-teknologier har revolusjonert feltet genomredigering. Imidlertid er det fortsatt en stor flaskehals å finne og isolere ønsket kimlinjeredigering. Derfor beskriver denne protokollen en robust metode for raskt å screene F0 CRISPR-injisert sebrafisksæd for germline-redigeringer ved bruk av standard PCR, restriksjonsfordøyelse og gelelektroforeseteknikker.

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Denne måneden i JoVE utgave 192 CRISPR-CAS9 sebrafisk sæd-DNA genomisk redigering
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