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Journal / Biology / マウス卵巣エピゲノムとトランスクリプトームの細胞特異的ペアインプロゲーション…
JoVE Journal
Biology

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JoVE Journal Biology
マウス卵巣エピゲノムとトランスクリプトームの細胞特異的ペアインプロゲーション
Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4
Journal (Biology)
Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4
Immunostaining of Mouse Stria Vascularis and Tissue Mounting (Video) | JoVE
Journal (Biology)
Immunostaining of Mouse Stria Vascularis and Tissue Mounting (Video) | JoVE
 
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マウス卵巣エピゲノムとトランスクリプトームの細胞特異的ペアインプロゲーション

Article DOI: 10.3791/64765-v • 12:25 min • February 24th, 2023
February 24th, 2023 •
*1,2,3, *1,3, 1, 2, 2, 4, 2,3, 1,3
1Aging and Metabolism Research Program, Oklahoma Medical Research Foundation, 2Genes & Human Disease Research Program, Oklahoma Medical Research Foundation, 3Oklahoma City Veterans Affairs Medical Center, 4Nutrition College, Federal University of Pelotas
* These authors contributed equally

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このプロトコルでは、翻訳リボソームアフィニティー精製(TRAP)法と特定の細胞タイプでタグ付けされた核の単離(INTACT)法が、Cyp17a1-Creマウス系統に交配されたNuTRAPマウスモデルを使用して、細胞特異的卵巣トランスクリプトームとエピゲノムのペアインプロゲーション用に最適化されました。

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今月のJoVE、第192号、
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