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JoVE Journal Bioengineering
Testing the In Vitro and In Vivo Efficiency of mRNA-Lipid Nanoparticles Formulated by Microfluidic Mixing
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Testing the In Vitro and In Vivo Efficiency of mRNA-Lipid Nanoparticles Formulated by Microfluidic Mixing

Article DOI: 10.3791/64810-v • 08:55 min • January 20th, 2023
January 20th, 2023 •
1, 1, 1, 1, 1
1Department of Bioengineering, University of Pennsylvania

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Here, a protocol for formulating lipid nanoparticles (LNPs) that encapsulate mRNA encoding firefly luciferase is presented. These LNPs were tested for their potency in vitro in HepG2 cells and in vivo in C57BL/6 mice.

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Bioengineering In Vitro Efficiency In Vivo Efficiency LNPs MRNA Cargo Nucleases Degradation Intracellular Delivery Ionizable Lipid Phospholipid Cholesterol Lipid-PEG Conjugate Firefly Luciferase Transfection Efficiency HepG2 Cells Bioluminescent Plate-based Assay C57BL/6 Mice Intravenous Injection Lateral Tail Vein Whole-body Bioluminescence Imaging In Vivo Imaging System
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