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Biochemistry

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JoVE Journal Biochemistry
Sondering af RNA-struktur med dimethylsulfatmutationsprofilering med sekventering in vitro og i celler
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Sondering af RNA-struktur med dimethylsulfatmutationsprofilering med sekventering in vitro og i celler

Article DOI: 10.3791/64820-v • 10:34 min • December 9th, 2022
December 9th, 2022 •
1, 1
1Department of Microbiology, Harvard Medical School

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Protokollen giver instruktion til modificering af RNA med dimethylsulfat til mutationsprofileringseksperimenter. Det omfatter in vitro og in vivo sondering med to alternative biblioteksforberedelsesmetoder.

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