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Journal / Biochemistry / Sondering av RNA-struktur med…
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Biochemistry

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JoVE Journal Biochemistry
Sondering av RNA-struktur med dimetylsulfatmutationsprofilering med sekvensering in vitro och i celler
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Sondering av RNA-struktur med dimetylsulfatmutationsprofilering med sekvensering in vitro och i celler

Article DOI: 10.3791/64820-v • 10:34 min • December 9th, 2022
December 9th, 2022 •
1, 1
1Department of Microbiology, Harvard Medical School

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Protokollet ger instruktioner för modifiering av RNA med dimetylsulfat för mutationsprofileringsexperiment. Den inkluderar in vitro - och in vivo-sondering med två alternativa biblioteksberedningsmetoder.

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