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Bioluminescent Monitoring of Graft Survival in an Adoptive Transfer Model of Autoimmune Diabetes in Mice
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Bioluminescent Monitoring of Graft Survival in an Adoptive Transfer Model of Autoimmune Diabetes in Mice

Article DOI: 10.3791/64836-v • 10:03 min • November 18th, 2022
November 18th, 2022 •
1, 2, 2, 1
1Section for Islet Cell and Regenerative Biology, Joslin Diabetes Center, Harvard Medical School, 2Section for Immunology, Joslin Diabetes Center, Harvard Medical School

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This protocol describes a straightforward and minimally invasive method for transplanting and imaging NIT-1 cells in non-obese diabetic (NOD)-severe combined immunodeficient mice challenged with splenocytes purified from spontaneously diabetic NOD mice.

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Bioluminescent Monitoring Graft Survival Adoptive Transfer Model Autoimmune Diabetes Mice Mechanisms Of Protection Therapeutic Targets Beta Cell Replacement Type One Diabetes Low Cost Simplicity Cell Culture Transplant Imaging Methods Invasiveness Mouse Techniques Tail Vein Injection Demonstration By An Expert Anesthetized Mouse Dorsal Side Electric Shaver Knockdown Chamber Isoflurane Nose Cone Isopropyl Alcohol Prep Pad Cell Suspension Sterile Syringe 26 Gauge Needle Curved Forceps
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