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Visualizing DNA Damage Repair Proteins in Patient-Derived Ovarian Cancer Organoids via Immunofluorescence Assays

Article DOI: 10.3791/64881-v • 04:07 min • February 24th, 2023
February 24th, 2023 •
1, 1, 1, 1, 1, 1,2, 1, 1
1Washington University in St. Louis, 2University of California San Francisco

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Summary
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The present protocol describes methods for evaluating DNA damage repair proteins in patient-derived ovarian cancer organoids. Included here are comprehensive plating and staining methods, as well as detailed, objective quantification procedures.

Tags

DNA Damage Repair Proteins Patient-derived Ovarian Cancer Organoids Immunofluorescence Assays DNA Damage Response Ovarian Cancer Biomarkers Targeted Therapy Antigen Examination Quick Protocol Chemotherapeutic Resistance PARP Inhibitor Resistance 3D Cultures Organoids Fixing Organoids Permeabilization Buffer Staining Buffer Primary Antibodies
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