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Visualization of Metabolites Identified in the Spatial Metabolome of Traditional Chinese Medicine Using DESI-MSI
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Visualization of Metabolites Identified in the Spatial Metabolome of Traditional Chinese Medicine Using DESI-MSI

Article DOI: 10.3791/64912-v • 08:02 min • December 16th, 2022
December 16th, 2022 •
1,4, 3, 3, 1, 1, 1,2
1Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, 2Tianfu Chinese Medicine Innovation Harbour, Chengdu University of Traditional Chinese Medicine, 3School of Medical Technology, Chengdu University of Traditional Chinese Medicine, 4China Resources Sanjiu (Ya’an) Pharmaceutical Co., Ltd.

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Summary
Automatic Translation

In this study, a series of methods are presented to prepare DESI-MSI samples from plants, and a procedure of DESI assembly installation, MSI data acquisition, and processing is described in detail. This protocol can be applied in several conditions for acquiring spatial metabolome information in plants.

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Visualization Metabolites Spatial Metabolome Traditional Chinese Medicine DESI-MSI Plant Samples Imaging Cost-effective Method Dry Tissues Nitrogen Blow In Cryosectioned Cryostat Microtome Imprinting Expensive Machine Signal Intensity Limitations Spatial Metabolomics Toxicants Distribution Abiotic Stress Biotic Stress Salvia Miltiorrhiza Plant Cross-sectional Thickness Adhesion Microscope Glass Slide Double-sided Tape Sealing Film Freeze Sample Air Vacuum Analysis Cold Storage Room Temperature Desiccator
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