JoVE Journal
Biology
Biology
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凍結保存された上皮陰窩からの子豚腸3Dオルガノイドの培養と細胞単分子膜の樹立
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Article
DOI:
10.3791/64917-v
•
08:19 min
•
February 10th, 2023
凍結保存された上皮陰窩からの子豚腸3Dオルガノイドの培養と細胞単分子膜の樹立
February 10th, 2023
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Chapters
Summary
Please note that all translations are automatically generated.
Click here for the English version.
ここでは、凍結保存された上皮陰窩からブタ腸管3Dオルガノイドを培養するためのプロトコルを記載する。また、3Dオルガノイド由来の細胞単分子膜を確立し、上皮細胞の頂端側へのアクセスを可能にする方法についても説明します。