Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Standardization of Transfer across Labs between…
JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content.

JoVE Journal Immunology and Infection
Standardization of Transfer across Labs between Flow Cytometers for Detection of Lymphocytes in Japanese Encephalitis Vaccinated Children
Detection of Neutralization-sensitive Epitopes in Antigens Displayed on Virus-Like Particle (VLP)-Based Vaccines Using a Capture Assay
Journal (Immunology and Infection)
Detection of Neutralization-sensitive Epitopes in Antigens Displayed on Virus-Like Particle (VLP)-Based Vaccines Using a Capture Assay
Rearing Ixodes scapularis, the Black-legged Tick: Feeding Immature Stages on Mice
Journal (Immunology and Infection)
Rearing Ixodes scapularis, the Black-legged Tick: Feeding Immature Stages on Mice
 

Standardization of Transfer across Labs between Flow Cytometers for Detection of Lymphocytes in Japanese Encephalitis Vaccinated Children

Article DOI: 10.3791/64949-v • 06:22 min • February 10th, 2023
February 10th, 2023 •
*1, *2,3, *2,3, 1, 1, 2,3, 2,3
1Center of Excellence (Beijing), Becton Dickinson Medical Devices (Shanghai) Co Ltd, 2Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, 3Research Unit of Critical Infection in Children, 2019RU016, Chinese Academy of Medical Sciences
* These authors contributed equally

Chapters

Summary
Automatic Translation

In this study, a method was developed to facilitate the transfer of experimental settings and analysis templates between two flow cytometers in two laboratories for the detection of lymphocytes in Japanese encephalitis-vaccinated children. The standardization method for the flow cytometer experiments will allow research projects to be effectively conducted in multiple centers.

Tags

Standardization Transfer Labs Flow Cytometers Detection Lymphocytes Japanese Encephalitis Vaccinated Children Mutual Accreditation Consistency Research Projects Multiple Centers Time Consumption Automation Data Analysis Configuration Software Base Configurations Folder Parameter Name FETC Detector 530/30 Experiment Performance Check Beads Cytometer A Setup Software Browser Window Worksheet Unstained Sample
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter