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Journal / Biochemistry / Single-Molecule Imaging of Lateral Mobility and…
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Biochemistry

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JoVE Journal Biochemistry
Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence (TIRF) Microscopy
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Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence (TIRF) Microscopy

Article DOI: 10.3791/64970-v • 08:55 min • February 17th, 2023
February 17th, 2023 •
1, 1
1Biophysics Department, Institute of Biomaterials and Biomolecular Systems, University of Stuttgart

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Summary
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This protocol describes how to use TIRF microscopy to track individual ion channels and determine their activity in supported lipid membranes, thereby defining the interplay between lateral membrane movement and channel function. It describes how to prepare the membranes, record the data, and analyze the results.

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Single-molecule Imaging Lateral Mobility Ion Channel Activity Lipid Bilayers Total Internal Reflection Fluorescence Microscopy TIRF Microscopy Membrane Diffusion Fluorescent Labeling Protein Channel Organization And Function DPhPC Stock Solution Lipid Oxidation Argon Or Nitrogen Gas Organic Solvent Evaporation Silicone Oil Solution Lipid Concentration Glass Coverslips
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