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Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e
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Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

Article DOI: 10.3791/64977-v • 07:31 min • February 17th, 2023
February 17th, 2023 •
1,2, 3, 1,2, 4, 1,2, 1,2
1Key Laboratory of Brain, Cognition and Education Science, Ministry of Education, 2Institute for Brain Research and Rehabilitation, Guangdong Key Laboratory of Mental Health and Cognitive Science, South China Normal University, 3Institute of Modern Aquaculture Science and Engineering, Guangdong Provincial Engineering Technology Research Center for Environmentally Friendly Aquaculture, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, School of Life Sciences, South China Normal University, 4Department of Pathology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences

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Summary
Automatic Translation

This protocol describes how to perform efficient adenine base editing without PAM limitation to construct a precise zebrafish disease model using zSpRY-ABE8e.

Tags

PAM-Less Base Editing ZSpRY-ABE8e Zebrafish Modeling Human Genetic Disease Single-nucleotide Variants Adenine To Guanine Base Conversion Intermutation PAN Restriction Zebrafish Models Disease Mechanisms Drug Screening Micropipette Puller System Glass Capillaries Program Settings Pulled Capillaries Preservation ZSpRY-ABE8e MRNA Easy Edit SgRNA GRNA Design NRN PAM Sequence Target Adenine Nucleotide
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