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JoVE Journal Immunology and Infection
The Superficial Inferior Epigastric Artery Axial Flap to Study Ischemic Preconditioning Effects in a Rat Model
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The Superficial Inferior Epigastric Artery Axial Flap to Study Ischemic Preconditioning Effects in a Rat Model

Article DOI: 10.3791/64980-v • 09:07 min • January 27th, 2023
January 27th, 2023 •
*1,2,3,5, *1,2,4,5, 1,2, 1,2,5, 1,2,5,6,7, 3, 2,5,6, 1,2,5,6, 2,4, 1,2,4,5, 1,2,4,5, 1,2,5,6,8
1Vascularized Composite Allotransplantation Laboratory, Massachusetts General Hospital, 2Harvard Medical School, 3Department of Plastic, Reconstructive and Aesthetic Surgery, Rennes University Hospital Center (CHU de Rennes), University of Rennes 1, 4Plastic Surgery Research Laboratory, Massachusetts General Hospital, 5Shriners Children's Boston, 6Center for Engineering in Medicine and Surgery, Massachusetts General Hospital, 7Department of Plastic Surgery and Hand Surgery, University Medical Center Utrecht, 8Department of Plastic, Reconstructive, and Aesthetic Surgery, Groupe Almaviva Santé, Clinique de l'Alma
* These authors contributed equally

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Summary
Automatic Translation

This protocol describes harvesting, suturing, and monitoring fasciocutaneous flaps in rats that allow for good visualization and manipulation of blood flow through the superficial inferior epigastric vessels by means of clamping and ligating the femoral vessels. This is critical for studies involving ischemic preconditioning.

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Superficial Inferior Epigastric Artery Axial Flap Ischemic Preconditioning Rat Model Surgical Protocol Fasciocutaneous Flaps Femoral Vessels Ischemia Reperfusion Injuries Flap Survivability Reconstructive Surgery Free Flap Reconstruction Pedicled Flap Reconstruction Flap Vulnerability Animal Sedation Abdominal Shaving Hair Removal Cream Sterile Skin Pen Inguinal Crease Incision Marking
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