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JoVE Journal Bioengineering
Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids
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Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids

Article DOI: 10.3791/65121-v • 10:42 min • May 12th, 2023
May 12th, 2023 •
*1, *1, 1, 1, 1, 1
1Shu Chien-Gene Lay Department of Bioengineering, University of California San Diego
* These authors contributed equally

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Summary
Automatic Translation

We present a protocol to directly visualize metabolic activities in cells regulated by amino acids using deuterium-oxide (heavy water D2O) probed stimulated Raman scattering (DO-SRS) microscopy, which is integrated with two-photon excitation fluorescence microscopy (2PEF).

Tags

Bioorthogonal Chemical Imaging Cell Metabolism Aromatic Amino Acids Label-free Imaging Technique Raman Imaging Two Photon Fluorescence Second Harmonic Generation Subcellular Resolution Metabolic Dysfunction Aging Cancer Non-destructive Imaging Technology Minimal Sample Preparation High Subcellular Resolution Home-built System DMEM Powder Double Distilled Water Deuterium Oxide FBS (fetal Bovine Serum) Penicillin And Streptomycin Phenylalanine Tryptophan Powder Methionine Threonine Syringe Filter
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