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JoVE Journal Biochemistry
High-Speed Magnetic Tweezers for Nanomechanical Measurements on Force-Sensitive Elements
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High-Speed Magnetic Tweezers for Nanomechanical Measurements on Force-Sensitive Elements

Article DOI: 10.3791/65137-v • 08:50 min • May 12th, 2023
May 12th, 2023 •
*1, *1, 1, 2,3, 2,3, 1,4
1Department of Physics, Pohang University of Science and Technology (POSTECH), 2School of Biological Sciences, Seoul National University, 3Institute for Molecular Biology and Genetics, Seoul National University, 4School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH)
* These authors contributed equally

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Here, we describe a high-speed magnetic tweezer setup that performs nanomechanical measurements on force-sensitive biomolecules at the maximum rate of 1.2 kHz. We introduce its application to DNA hairpins and SNARE complexes as model systems, but it will be also applicable to other molecules involved in mechanobiological events.

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High-speed Magnetic Tweezers Nanomechanical Measurements Force-sensitive Elements Biomolecules Structural Changes High Resolution Physiologically Relevant Forces Nucleic Acids Proteins Real Time Monitoring Malfunctions Bearing Proteins Mechanosensitive Proteins Insight On Working Mechanisms Alignment And Calibration Preparation Of Molecular Constructs Inverted Microscope Anti-vibration Optical Table High-speed CMOS Camera Frame Grabber Motorized Linear Stage Translation Stage Magnet Manipulation 3D Rotation Rotary Stepper Motor Belt And Pulley System Acrylic Holder
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