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08:37 min
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March 10, 2023
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The present protocol describes how to use oil red O to dye lipid droplets (LDs), calculate the size and number of LDs in a fatty acid-induced fatty hepatocyte model, and use BODIPY 493/503 to observe the process of small LDs fusing into large LDs by live cell imaging.
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Cite this Article
Yang, J., Kang, F., Wei, A., Lu, W., Zhang, X., Han, L. Evaluation of Lipid Droplet Size and Fusion in Bovine Hepatic Cells. J. Vis. Exp. (193), e65234, doi:10.3791/65234 (2023).
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