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06:45 min
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June 02, 2023
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Transcript
Automatically generated
私たちの研究は主に線溶酵素の精製に関するものです。本研究では、線溶酵素の簡便な精製法の確立を試みる。本研究では、線溶酵素のアルギニンまたはリジン最親和性精製系を初めて報告する。
従来の精製方法と比較して、これはSipunculus nudusからの線溶酵素の精製のための簡単で安価で効率的なアプローチです。このプロトコルの開発は、Sipunculus nudusおよび他の同様の薬剤からの線維素溶解酵素のさらなる利用にとってより重要である。
Summary
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ここでは、 Sipunculus nudus 由来の線溶酵素の簡便、安価、効率的なアフィニティー精製法を紹介します。
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Cite this Article
Tang, M., Lin, H., Hu, C., Yan, H. Affinity Purification of a Fibrinolytic Enzyme from Sipunculus nudus. J. Vis. Exp. (196), e65631, doi:10.3791/65631 (2023).
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