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Journal / Biology / Analisi immunoblot
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Biology

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JoVE Journal Biology
Analisi immunoblot
Measuring In Vitro ATPase Activity for Enzymatic Characterization
Journal (Biology)
Measuring In Vitro ATPase Activity for Enzymatic Characterization
RNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling
Journal (Biology)
RNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling
 
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Analisi immunoblot

Article DOI: 10.3791/759-v • 16:01 min • June 20th, 2008
June 20th, 2008 •
1, 2
1UVP, LLC, 2Proteomic Center, Keck Graduate Institute of Applied Life Sciences

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Immunoblotting (Western blotting) è un test rapido e sensibile per la rilevazione e la caratterizzazione di proteine ​​che funziona sfruttando la specificità inerenti antigene-anticorpo riconoscimento. Questo video fornisce protocolli per la separazione delle proteine, le proteine ​​assorbente su membrane, immunoprobing, e la visualizzazione utilizzando substrati cromogeni o chemiluminescente.

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Protocolli di base Numero 16 Current Protocols Wiley immunoblotting Biochimica Western Blotting substrati cromogeni chemiluminescente substrati il rilevamento delle proteine.
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