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JoVE Journal
Biology

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Method for Culture of Early Chick Embryos ex vivo (New Culture)
 
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Method for Culture of Early Chick Embryos ex vivo (New Culture)

Article DOI: 10.3791/903-v 08:45 min October 20th, 2008
October 20th, 2008

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Summary

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Click here for the English version.

This video demonstrates New culture, a method by which chick embryos are cultured outside the egg for up to 24 hr. This method enables one to study early development (primitive streak to 14 som.), a period corresponding to E7-9 in mouse. Applications of this technique include electroporation, in situ hybridization and immunohistochemistry.

Tags

Chick Embryo Culture Early Embryonic Development Brain Formation Neural Tube Somite Heart Primordia Gene Function Analysis Growth Factor Coated Beads FGFs BMPs Whole Mount In Situ Hybridization Immunohistochemistry Explantation Glass Ring Albumine Pool Culture Dish Sealing Humid Chamber Troubleshooting Guide
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