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Calcium Imaging of Cortical Neurons using Fura-2 AM
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Calcium Imaging of Cortical Neurons using Fura-2 AM

Article DOI: 10.3791/1067-v • 12:16 min • January 19th, 2009
January 19th, 2009 •
1, 2
1Department of Neurobiology, Stanford University, 2Department of Neurobiology, Stanford University School of Medicine

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Calcium signals play a key role in many cellular processes including gene expression, survival and differentiation. Here we demonstrate how to perform calcium imaging using Fura-2 AM. Calcium imaging is a valuable tool to study the regulation of intracellular calcium in real time and its regulation of signaling cascades.

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Calcium Imaging Cortical Neurons Fura-2 AM Calcium Signals Calcium Indicator Dyes BAPTA-based Organic Molecules Ratio-metric Dyes Single-wavelength Dyes Fluo-4 Intracellular Calcium Concentration Fluorescence Emission Excitation Spectra Calcium Binding Calcium Elevations Excitable Cells
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