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Photo-Induced Cross-Linking of Unmodified Proteins (PICUP) Applied to Amyloidogenic Peptides
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Photo-Induced Cross-Linking of Unmodified Proteins (PICUP) Applied to Amyloidogenic Peptides

Article DOI: 10.3791/1071-v • 08:40 min • January 12th, 2009
January 12th, 2009 •
1, 1, 2,3
1Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles, 2Brain Research Institute, Molecular Biology Institute, University of California, Los Angeles, 3Department of Neurology, University of California, Los Angeles

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Summary
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Photo-induced cross-linking of unmodified proteins (PICUP) allows characterization of oligomer size distribution in metastable protein mixtures. We demonstrate application of PICUP to three representative amyloidogenic peptides the 40- and 42-residue forms of amyloid β-protein, and calcitonin, and a control peptide growth-hormone releasing factor.

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Photo-Induced Cross-Linking Of Unmodified Proteins PICUP Amyloidogenic Peptides Protein Misfolding Diseases Alzheimer's Disease Parkinson's Disease Huntington's Disease Hereditary Amyotrophic Lateral Sclerosis Type 2 Diabetes Oligomer Size Distribution Classical Methods Electrophoresis Chromatography Fluorescence Dynamic Light Scattering Metastable Mixtures Covalent Cross-linking Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) Size-exclusion Chromatography (SEC) Visualization Quantitative Analysis Assembly Monitoring Sequence Modifications Oligomerization Mechanism
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