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Photo-induzierte Vernetzung von unmodifizierten Proteins (picup) zu amyloidogene Peptide Applied
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Photo-induzierte Vernetzung von unmodifizierten Proteins (picup) zu amyloidogene Peptide Applied

Article DOI: 10.3791/1071-v • 08:40 min • January 12th, 2009
January 12th, 2009 •
1, 1, 2,3
1Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles, 2Brain Research Institute, Molecular Biology Institute, University of California, Los Angeles, 3Department of Neurology, University of California, Los Angeles

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Photo-induzierte Quervernetzung von unmodifizierten Proteine ​​(picup) ermöglicht die Charakterisierung des Oligomers Größenverteilung in metastabilen Protein-Mischungen. Wir zeigen, Anwendung picup zu drei Vertreter amyloidogene Peptide der 40 - und 42-Rückstand bildet der Amyloid-β-Protein und Calcitonin, und eine Kontrollgruppe Peptid Wachstumshormon-Releasing-Faktor.

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Vernetzung picup Amyloid-β-Protein Oligomer Amyloid- Protein-Montage
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