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社区环境转录从海洋微生物基因表达分析
 
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社区环境转录从海洋微生物基因表达分析

Article DOI: 10.3791/1086-v 13:51 min February 18th, 2009
February 18th, 2009

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Summary

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我们提出了一个方法生成环境的mRNA的cDNA。在一般情况下,总RNA是第一次从环境中收集,rRNA的选择性去除,mRNA的选择性扩增,并从丰富的mRNA池合成cDNA测序。使用标准的生物信息学技术确定基因表达,恢复序列,可以注明。

Tags

微生物学,24期,转录,浮游细菌,基因,微生物群落的基因表达
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