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Monitoring Plant Hormones During Stress Responses
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Monitoring Plant Hormones During Stress Responses

Article DOI: 10.3791/1127-v • 11:01 min • June 15th, 2009
June 15th, 2009 •
1, 1
1Department of Biology, University of Texas

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A simple method is provided that allows for the rapid extraction and analysis of multiple plant hormones from small tissue samples. The procedure uses vapor phase extraction as the solemn purification step. Samples are analyzed by GC/MS with chemical ionization that produces mainly (M+1)+ ions.

Tags

Plant Hormones Stress Responses Environmental Stimuli Insect Herbivory Pathogen Infection Drought Stress Jasmonic Acid (JA) Salicylic Acid (SA) Abscisic Acid (ABA) Temporal Monitoring Spatial Monitoring Vapor Phase Extraction Gas Chromatography/mass Spectrometry (GC/MS) Analysis Quantification Internal Standards
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