Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

Channelrhodopsin2 Mediated Stimulation of Synaptic Potentials at Drosophila Neuromuscular Junctions
 

Channelrhodopsin2 Mediated Stimulation of Synaptic Potentials at Drosophila Neuromuscular Junctions

Article DOI: 10.3791/1133-v 09:13 min March 16th, 2009
March 16th, 2009

Chapters

Summary

This procedure uses a blue light-activated algal channel and cell-specific genetic expression tools to evoke synaptic potentials with light pulses at the neuromuscular junction (NMJ) in Drosophila larvae. This technique is an inexpensive and easy-to-use alternative to suction electrode stimulation for synaptic physiology studies in research and teaching laboratories.

Tags

Channelrhodopsin2 Stimulation Synaptic Potentials Drosophila Neuromuscular Junctions Larval Preparation Excitatory Junctional Potentials Suction Electrodes Remote Stimulation Motor Neurons GAL4-UAS System Electrophysiology Rig Blue Light Neurophysiology Teaching Labs
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter