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Journal / Biology / Homemade Site Riktad mutagenes av Whole Plasmids
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Homemade Site Riktad mutagenes av Whole Plasmids
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Homemade Site Riktad mutagenes av Whole Plasmids

Article DOI: 10.3791/1135-v • 07:12 min • May 11th, 2009
May 11th, 2009 •
1, 2
1Department of Biology, Johannes Gutenberg-University Mainz, Germany, 2Proteomics division, AlPlanta, Neustadt an der Weinstrasse, Germany

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Site riktad mutagenes av hela plasmider är ett enkelt sätt att skapa lite olika varianter av ett original plasmid. Här visar vi ett enkelt och kostnadseffektivt sätt att introducera basera ersättningar till en plasmid med vanliga reagenser.

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Grundläggande protokoll Site riktad mutagenes mutagenes mutation Plasmid termocykling PCR Pfu-polymeras Dpn1 kostnadsbesparing
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