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Visualisering antigenspecifikt CD4 + T celler med MHC klass II Tetramers
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Visualisering antigenspecifikt CD4 + T celler med MHC klass II Tetramers

Article DOI: 10.3791/1167-v • 15:43 min • March 6th, 2009
March 6th, 2009 •
1, 2, 3, 2
1Tetramer Core Laboratory, Benaroya Research Institute, 2Kwok Laboratory, Benaroya Research Institute, 3Nepom Laboratory, Benaroya Research Institute

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Detta förfarande visar på rening och in vitro utbyggnad av antigen CD4 + T-celler från hela perifert blod och visualisering med hjälp av MHC klass II tetramers. Tetramers tillåter direkt visualisering av T-celler med en enda antigenspecificitet och definieras MHC klass II begränsning.

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Immunologi CD4 + T-cell MHC klass II tetramers perifera mononukleära celler in vitro expansion flödescytometri
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