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Proteômica para identificar proteínas Interagindo com P2X2 Canais Ligand-Gated Cation
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Proteômica para identificar proteínas Interagindo com P2X2 Canais Ligand-Gated Cation

Article DOI: 10.3791/1178-v • 16:37 min • May 18th, 2009
May 18th, 2009 •
1, 2, 3, 1
1Department of Physiology, David Geffen School of Medicine, University of California, Los Angeles, 2Department of Anesthesiology, David Geffen School of Medicine, University of California, Los Angeles, 3Department of Anesthesiology, Medicine and Physiology, David Geffen School of Medicine, University of California, Los Angeles

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Nós descrevemos um protocolo simples para identificar proteínas cerebrais que se ligam ao terminal de todo o comprimento C da ATP-gated P2X2 receptores. A extensão e aplicação sistemática desta abordagem para todos os receptores P2X deverá levar a uma melhor compreensão da sinalização do receptor P2X.

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Neurociência Edição 27 Pull down proteína recombinante GST cérebro de ratos espectrometria de massa as interações proteína P2X2 complexo macromolecular canal receptor purinérgicos
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