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Cellular Toxicity of Nanogenomedicine in MCF-7 Cell Line: MTT assay

Article DOI: 10.3791/1191-v • 19:35 min • April 3rd, 2009
April 3rd, 2009 •
1,2, 1,3, 1, 2, 1,3
1Research Center for Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tabriz University (Medical Sciences), 2Gifted and Talented Students Office, Educational Development Center, Tabriz University (Medical Sciences), 3School of Advanced Biomedical Sciences, Tabriz University (Medical Sciences)

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The MTT assay is an easy and reproducible colorimetric assay for evaluation of cell viability based on reduction of yellow MTT and production of water insoluble purple formazan. Here, the viability of MCF-7 cells upon treatment of nanogenomedicine has been evaluated.

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Cellular Toxicity Nanogenomedicine MCF-7 Cell Line MTT Assay Cytotoxicity Short Interfering RNA Antisense Gene-based Medicine Carrier Colorimetric Approach Mitochondrial Dehydrogenase Enzymes Nanopolyplexes Water Soluble Yellow MTT Formazan Dimethylsulfoxide Sornson's Buffer PH 10.5 Spectrophotometry
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