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Cultura primária de Miócitos Coração Adulto Rat

Article DOI: 10.3791/1308-v • 11:44 min • June 16th, 2009
June 16th, 2009 •
1, 1,2
1Department of Physiology and Cellular Biophysics, Columbia University, 2Department of Pharmacology, Columbia University

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Neste artigo, descrevemos uma forma típica para isolar e cultura miócitos adulto coração de rato. Colagenase e protease são usadas para digerir e isolar miócitos único. Miócitos cultivadas seguir este protocolo de responder às necessidades mais experiência.

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Biologia Celular Edição 28 Rat o coração o isolamento cultura primária cardiologia
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