This video demonstrates two methods for preparing and placing beads, which have been coated with growth factor, on explants of the developing cerebral cortex. These beads can be used to induce spatially restricted gene expression on developing neural tissue such as forebrain explants. Methods are given for using both Affi-Gel beads and heparin acryllic beads.
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Currle, D. S., Kolski-Andreaco, A., Monuki, E. S. Growth Factor-Coated Bead Placement on Dorsal Forebrain Explants. J. Vis. Exp. (2), e134, doi:10.3791/134 (2007).
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The authors have nothing to disclose.