Mouse Dorsal Forebrain Explant Isolation

Biology

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Summary

This video demonstrates the protocol for isolating and culturing explants of the mouse forebrain from embyonic day 12 mice. Procedures for removal of the uterus, embryos from uterus, and dissection of embryos are given. In addition the methodology for transferring these explants onto specialized membranes on which they are cultured is demonstrated. The development of the forebrain can be studied in vitro using this preparations as well as changes in gene expression.

Cite this Article

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Currle, S., Kolski-Andreaco, A., Monuki, E. S. Mouse Dorsal Forebrain Explant Isolation. J. Vis. Exp. (2), e135, doi:10.3791/135 (2007).

Abstract

Disclosures

The authors have nothing to disclose.

Comments

2 Comments

  1. What kind of membrane is this? Will neurons grow out of the explant?

    Reply
    Posted by: Anonymous
    July 22, 2008 - 3:37 AM
  2. Whatman Nucleopore filter. Don't know about the neurons (we don't use this system for neuronal process outgrowth). ESM

    Reply
    Posted by: Anonymous
    July 23, 2008 - 2:18 AM

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