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Large Insert Environmental Genomic Bibliothek Produktion
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Large Insert Environmental Genomic Bibliothek Produktion

Article DOI: 10.3791/1387-v • 21:00 min • September 23rd, 2009
September 23rd, 2009 •
1, 1, 1, 1, 1
1Department of Microbiology and Immunology, University of British Columbia - UBC

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Bau eines Fosmid Bibliothek mit Umwelt-genomischer DNA aus der vertikalen Tiefe Kontinuum einer saisonal hypoxischen Fjord isoliert beschrieben. Der resultierende Klon-Bibliothek ist in 384-Well-Platten aufgenommen und archiviert für Downstream-Sequenzierung und funktionelle Screening durch die Anwendung eines automatisierten Kolonie Kommissioniersystem.

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Grundlegende Protokolle Ausgabe 31 Umwelt-Genomik metagenomische genomische DNA große einfügen Bibliothek Fosmid Phagen-Verpackung automatisierte Kommissionierung Kolonie
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