This protocol demonstrates the dissection technique used for isolating imaginal discs from drosophila larvae at the 3rd instar stage. Methods for fixing the tissue after saying and removing the wing, leg, and eye discs are demonstrated directly on a microscope slide for subsequent visualization.
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Purves, D. C., Brachmann, C. Dissection of Imaginal Discs from 3rd Instar Drosophila Larvae. J. Vis. Exp. (2), e140, doi:10.3791/140 (2007).
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The authors have nothing to disclose.