Dissection of Imaginal Discs from 3rd Instar Drosophila Larvae

Biology

Your institution must subscribe to JoVE's Biology section to access this content.

Fill out the form below to receive a free trial or learn more about access:

Welcome!

Enter your email below to get your free 10 minute trial to JoVE!





We use/store this info to ensure you have proper access and that your account is secure. We may use this info to send you notifications about your account, your institutional access, and/or other related products. To learn more about our GDPR policies click here.

If you want more info regarding data storage, please contact gdpr@jove.com.

 

Summary

This protocol demonstrates the dissection technique used for isolating imaginal discs from drosophila larvae at the 3rd instar stage. Methods for fixing the tissue after saying and removing the wing, leg, and eye discs are demonstrated directly on a microscope slide for subsequent visualization.

Cite this Article

Copy Citation | Download Citations

Purves, D. C., Brachmann, C. Dissection of Imaginal Discs from 3rd Instar Drosophila Larvae. J. Vis. Exp. (2), e140, doi:10.3791/140 (2007).

Abstract

Disclosures

The authors have nothing to disclose.

Comments

2 Comments

  1. It would be good if you can show some pictures of imaginal discs

    Reply
    Posted by: Anonymous
    January 29, 2010 - 6:36 AM
  2. good

    Reply
    Posted by: naglaa e.
    April 21, 2013 - 12:49 AM

Post a Question / Comment / Request

You must be signed in to post a comment. Please or create an account.

Usage Statistics