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Isolation and Genetic Manipulation of Adult Cardiac Myocytes for Confocal Imaging
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Isolation and Genetic Manipulation of Adult Cardiac Myocytes for Confocal Imaging

Article DOI: 10.3791/1433-v • 12:16 min • September 17th, 2009
September 17th, 2009 •
1, 1, 1, 1, 1, 1
1Institute for Molecular Cell Biology, Universty of Saarland

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Summary
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Adult cardiac myocytes are primary cells that can be isolated from animal hearts and cultured for several days. Within this culture period adenoviral gene transfer can be used to express genetically encoded biosensors (GEBs) or fluorescent fusion proteins. Both approaches allow cellular investigations by means of confocal microscopy.

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Isolation Genetic Manipulation Adult Cardiac Myocytes Confocal Imaging Embryonic Cells Neonatal Muscle Cells Working Heart Cardiac Cellular Physiology Pathophysiology Pharmaceutical Investigations Transgenic Animal Models Long Term Culture Adenoviral Gene Transfer Genetically Encoded Biosensors Fluorescent Fusion Proteins Protein Overexpression Protein Downregulation RNAi Subcellular Structure Staining Golgi Visualization Confocal Imaging Z-stacks Reconstruction Cellular Cardiology
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