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JoVE Journal
Biology

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重新用四个转录因子的小鼠胚胎成纤维细胞诱导多能干细胞的产生,强力霉素诱导慢病毒转导系统
 
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重新用四个转录因子的小鼠胚胎成纤维细胞诱导多能干细胞的产生,强力霉素诱导慢病毒转导系统

Article DOI: 10.3791/1447-v 11:49 min November 13th, 2009
November 13th, 2009

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Summary

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Click here for the English version.

Stemgent阿霉素诱导小鼠TF慢病毒设置可以重新编程诱导多能干细胞(iPS细胞)的小鼠胚胎成纤维细胞(MEFs)。在这里,我们展示了鼠标重新编程转录因素Oct4的DOX诱导表达的协议,SOX2,KLF4和c - Myc基因产生的iPS殖民地,表达共同的MES多能性标志物。

Tags

发育生物学,第33期,重新编程,强力霉素,阿霉素,IPS,诱导多能干细胞,慢病毒,多能性,传导,干细胞
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