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Denaturierung Urea Polyacrylamidgelelektrophorese (Urea PAGE)
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Denaturierung Urea Polyacrylamidgelelektrophorese (Urea PAGE)

Article DOI: 10.3791/1485-v • 08:44 min • October 29th, 2009
October 29th, 2009 •
1, 2, 1
1School of Biological Sciences, Nanyang Technological University, Singapore - NTU, 2Singapore-MIT Alliance for Reserach and Technology (SMART)

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Denaturierung Harnstoff-Polyacrylamid-Gelelektrophorese wird verwendet, um Einzelstrang-DNA oder RNA getrennt bis zu einer Grenze von 500 Nukleotiden. Urea in Kombination mit Hitze denaturiert Proben und unstrukturierte Einzelstränge in der Gel-Matrix migrieren nach ihrem Molekulargewicht.

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Molecular Biology Ausgabe 32 DNA-und RNA-Analyse denaturierende Harnstoff-Polyacrylamid-Gelelektrophorese Protokolle
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