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Denaturering Urea polyakrylamidgelelektrofores (urea SIDA)

Article DOI: 10.3791/1485-v • 08:44 min • October 29th, 2009
October 29th, 2009 •
1, 2, 1
1School of Biological Sciences, Nanyang Technological University, Singapore - NTU, 2Singapore-MIT Alliance for Reserach and Technology (SMART)

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Denaturering urea polyakrylamidgelelektrofores används för att separera enda DNA eller RNA upp till en gräns på 500 nukleotider. Urea i kombination med värme denaturerar prover och ostrukturerade enstaka delar migrera inom gelmatrisen enligt deras molekylvikt.

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Molekylärbiologi DNA och RNA-analys denaturering urea polyakrylamidgelelektrofores protokoll
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