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Lentivirus הפקה
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Lentivirus הפקה

Article DOI: 10.3791/1499-v • 11:43 min • October 2nd, 2009
October 2nd, 2009 •
1, 1
1Diabetes Center, University of California, San Francisco - UCSF

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כדי להפוך את lentiviruses, וקטורים DNA הם transfected 293 לתוך תאים אנושיים. לאחר הקציר ריכוז supernatant, titer וירוס נקבעת על ידי ביטוי פלואורסצנציה עם cytometer זרימה.

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מיקרוביולוגיה גיליון 32 Lentivirus RNAi טיטרציה ויראלי transfection רטרווירוס cytometry זרימה וקטור מערכת מפוצלת shRNA.
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